ETUDE RECHERCHE Brain region-specific alterations of RNA editing in PDE8A mRNA in suicide decedents

Brain region-specific alterations of RNA editing in PDE8A mRNA in suicide decedents
Fabrice Chimienti ,1 Laurent Cavarec, 2, Laurent Vincent , 3 Nicolas Salvetat, 1 Victoria Arango, 4,5 Mark D. Underwood, 4,5 J. John Mann, 4, 5 Jean-François Pujol 1 & Dinah Weissmann  1

1 ALCEDIAG/ Sys2Diag, CNRS UMR 9005, Parc Euromédecine, Montpellier, France
2 Genomic Vision, Green Square, 80-84 rue des Meuniers, 92220, Bagneux, France
3 Commissariat à l’Energie Atomique, Fontenay aux Roses, France
4 Division of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, New York, NY, USA
5 Department of Psychiatry, Columbia University College of Physicians and Surgeons, New York, NY, USA

Translational Psychiatry volume 9, Article number: 91 (2019) 

Abstract

Phosphodiesterases (PDE) are key modulators of signal transduction and are involved in inflammatory cell activation, memory and cognition. There is a two-fold decrease in the expression of phosphodiesterase 8A (PDE8A) in the temporal cortex of major depressive disorder (MDD) patients. Here, we studied PDE8A mRNA-editing profile in two architectonically distinct neocortical regions in a clinically well-characterized cohort of age- and sex-matched non-psychiatric drug-free controls and depressed suicide decedents. By using capillary electrophoresis single-stranded conformational polymorphism (CE-SSCP), a previously validated technique to identify A-to-I RNA modifications, we report the full editing profile of PDE8A in the brain, including identification of two novel editing sites. Editing of PDE8A mRNA displayed clear regional difference when comparing dorsolateral prefrontal cortex (BA9) and anterior cingulate cortex (BA24). Furthermore, we report significant intra-regional differences between non-psychiatric control individuals and depressed suicide decedents, which could discriminate the two populations. Taken together, our results (i) highlight the importance of immune/inflammatory markers in major depressive disorder and suicide and (ii) establish a direct relationship between A-to-I RNA modifications of peripheral markers and A-to-I RNA editing-related modifications in brain. This work provides the first immune response-related brain marker for suicide and could pave the way for the identification of a blood-based biomarker that predicts suicidal behavior.

https://www.nature.com/articles/s41398-018-0331-3